Southern blotting is the type of blotting that is used to detect DNA while western blotting is used to find out protein. There are different types of blotting on the basis of detection of specific material, i.e., southern blotting, northern blotting, and western blotting. These molecules are pressed against the surface of an immobilized membrane which transfers the molecules onto the membrane. Here, minute particles will move faster than the bigger ones. In this technique, macromolecules like nucleic acids (DNA and RNA) and proteins in a mixture move through a slab of gel. in the serum, to find out defective proteins and used as a definite measure for herpes, hepatitis B, Lyme disease, and Creutzfeldt-Jacob disease, etc.īlotting is a technique that is used by scientists to separate different types of molecules from a mixture or sample. Western blotting is used to find the number of protein in a mixture, to detect the presence of HIV, virus, and bacteria, etc. Southern blotting is used in DNA detection, paternity testing, DNA fingerprinting, for victim identification, for identifying criminals, to find infectious agents and to identify mutation or gene rearrangement, etc. Labeling methods used in western blotting are the use of fluorescently labeled antibody or radiolabelling, chromogenic dyes or formation of diaminobenzidine, etc.ĭetection of light, Autoradiograph, and changes in color are used as detection methods in southern blotting.ĭetection methods in western blotting are changes in color and detection of light etc.

No any step like blocking is involved in southern blotting.ĭuring western blotting, nonspecific antibody sites are blocked on the nitrocellulose paper with the help of milk powder or bovine serum albumin (BSA).Ĭommon labeling methods that are used in southern blotting are the use of chromogenic dyes or radiolabelling or fluorescent labeling etc. Western Blotting follows electric transfer procedure.ĭuring the Southern blotting, the sample needs to be denaturated.ĭuring western blotting, the sample should be in its native state. Southern blotting follows capillary transfer procedure. SDS PAGE/ Polyacrylamide gel used to separate proteins in western blotting Primary and secondary antibodies are used as a probe.Īgarose gel electrophoresis is used in Southern blotting. It works on the principle of immunodetection method or antigen-antibody interaction.Ī single-stranded DNA or sometimes RNA is used as a probe. It works on the principle of hybridization. Western blotting is used to find out a specific amino acid or protein sequence. Southern blotting is used to find out a specific DNA sequence. Western blotting was developed by George Stark’s group at Stanford University in 1979. Southern blotting was developed by Edward M. ADVERTISEMENT Comparison Chart Southern BlottingĪ technique that is used to find out a specific DNA sequence in a given mixture is known as southern blotting.Ī technique that is used to find out a specific amino acids sequence of the protein in a given mixture is known as southern blotting.

Western blot vs elisa